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KMID : 1094720090140010027
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 1 p.27 ~ p.31
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Partial Characterization of Extracellular Polysaccharides Produced by Cyanobacterium Arthrospira platensis
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Lamia Trabelsi
Nour Houda M’sakni
Hatem Ben Ouada
Hassen Bacha
Sadok Roudesli
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Abstract
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In this study, the physico-chemical characteristics of extracellular polysaccharides (EPS) produced by Arthrospira platensis were evaluated. Elemental analysis and a bicinchoninic acid (BCA) reaction indicated that the EPS were heteropolysaccharides that contain carbohydrate (13%) and protein (55%) moieties. Analysis of the infrared spectrum and elemental analysis revealed the presence of a sulfate group (0.5%). The UV-visible spectrum showed high UV absorption at 190~230 nm and a shoulder at 260~280 nm. In addition, this spectrum indicated that EPS can form aggregates with mycosporine-like amino acids and/or scytonemin. Gas chromatography analysis of the carbohydrate portion of the EPS indicated that it was composed of seven neutral sugars: galactose (14.9%), xylose (14.3%), glucose (13.2%), frucose (13.2%), rhamnose (3.7%), arabinose (1%), and mannose (0.3%) and two uronic acids, galacturonic acid (13.5%) and glucuronic acid (0.9%).
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KEYWORD
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Arthrospira platensis, cyanobacteria, heteropolysaccharides, physico-chemical characterization
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